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Alginate-ionic liquid nanoparticles (ALNPs) were prepared by a modified layer-by-layer (LbL) method using two kinds of carboxylic groups, 2-carboxyethylsulfanilic acid (CESA) and 4-carboxybutylate-4-sulfanilamidosalicylic acid (CASA), respectively. The nanoparticles were characterized by several techniques and demonstrated good dispersibility and pH-responsive drug release property. The nanoparticles could absorb relatively more protons in the tumor acidic environment, resulting in sustained release of the encapsulated drug. Despite the high drug loading (20%), the nanoparticles were capable of yielding a high drug loading efficiency (∼80% for intact nanoparticles). In vivo studies showed that the drug-loaded nanoparticles were efficiently delivered to the tumor, where they released the drug in a pH-dependent manner. Compared with encapsulated free DOX, these nanoparticles were shown to have greater tumor inhibition rate. Importantly, the nanoparticles were successfully conjugated with biotinylated epidermal growth factor receptor-targeting peptide and further demonstrated specific binding to breast cancer (MCF-7) cells in vitro. These particles might be useful for tumor-targeted drug delivery.package com.planet_ink.coffee_mud.Commands;
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This study examined the role of corticotrophin-releasing factor (CRF) in the regulation of gonadotropin secretion and the involvement of CRF-1 receptor in the control of gonadotropin secretion. Intravenous administration of the CRF-1 receptor antagonist antalarmin was used to evaluate the role of CRF-1 in the control of luteinizing hormone (LH) secretion. We also infused exogenous CRF to examine the changes of gonadotropin secretion. Results indicate that antalarmin infusion (100 microg/h for 90 min) inhibited LH and follicle-stimulating hormone (FSH) secretion by 76.6+/-6.4% and 89.1+/-3.2%, respectively. Exogenous CRF infusion (150 microg/h for 90 min) stimulated LH and FSH by 2.4+/-1.1-fold and 4.3+/-1.2-fold, respectively. When CRF-1 antagonist was administrated 30 min before the CRF infusion, there was no difference in LH or FSH response to the exogenous CRF infusion. CRF decreased the secretion of LH and FSH after the removal of the hypothalamic-pituitary graft in castrated male rhesus monkeys. The level of CRF in the plasma of monkeys after castration was significantly decreased compared with that in normal monkeys. These results suggest that CRF plays an important role in the control of gonadotropin secretion in rhesus monkeys and CRF-1 receptor is involved in the negative feedback of gonadotropin secretion.Many people who have fixed term contracts at Christmas have complained that they receive repeated notification of their appointments well in advance – which means by the time they have got their hair cut they have missed their appointments.
The Notifications Directorate of the Business Services Authority has a website that allows people to check details of multiple notifications.
We have attached this letter to our comments in this important document about holiday pay which we are sending to